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KMID : 1098419950030030251
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Korean Journal of Medicinal Crop Science
1995 Volume.3 No. 3 p.251 ~ p.258
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Increment of Germanium Contents in Angelica keiskei Koidz . and Panax ginseng G . A . Meyer by In Vitro Propagation
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이만상/Lee, Man Sang
남궁승박/권태오/이종호/NamKoong, Seung Bak/Kwon, Tae Oh/Lee, Joong Ho
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Abstract
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This study was carried out to find optimum concentration of germanium compounds and pH of medium on the induction and growth of callus from A. keiskei and P. ginseng and to intend to increase Ge. absorption by calli while those calli were subculturing on MS medium. Callus from a. keiskei was rarely induced under light condition. Under dark condition, callus induction from A. keiskei was good up to 5ppm, retarded at 50ppm of $GeO_2$ 수식 이미지, or C. E. Ge. O., and rarely done at 100 ppm of $GeO_2$ 수식 이미지 but was somewhat well at 100 ppm of C. E. Ge. O. The induction and growth of callus was good in order of pH 5. 7 > pH 5. 4 > pH 6. 0 Under light condition, the growth of callus induced from P. ginseng was poor at $1{\sim}10\;ppm$ 수식 이미지 of $GeO_2$ 수식 이미지, or C. E. Ge. O., but shooting from callus occurred frequently. Under dark condtion, the growth of callus from A. keiskei was good up to 5 ppm of $GeO_2$ 수식 이미지, or C. E. Ge. O. and was rarely done at 50 ppm of $GeO_2$ 수식 이미지, but was somewhat well even at 100 ppm of C. E. Ge. O. Shooting from callus occurred frequently in a. keiskei, especially at pH 5.7. The growth of callus from P. ginseng was poor at 10 ppm of $GeO_2$ 수식 이미지, or 50 ppm of C. E. Ge. O. Under dark condition, the amount of Ge absorption by callus induced from A. keiskei was much higher than that from P. ginseng. The amount of Ge. absorption by callus treated with $GeO_2$ 수식 이미지, was higher than that treated with C. E. Ge. O.
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